Haynes Creek Preserve is primarily an island that lies between a canal and Haynes Creek and is also a launch point for the Lake Eustis Run Blueway Trail. It consists of wet deciduous forest with a small amount of upland. One can expect to hear Barred Owls and Pileated Woodpeckers while hiking the trails. During the summer nesting season, Prothonotary Warbler, Northern Parula and Red-Eyed Vireos are present. Other wildlife includes more than 20 species of butterflies and dragonflies, Race Runners, River Otters and American Alligators. Purple Gallinules and other waders can be found where the trails end overlooking Haynes Creek.
The Preserve features nature/hiking trails and picnic areas.